Temperature-Sensitive Mutants of Bacteriophage Mu
نویسندگان
چکیده
منابع مشابه
Temperature-sensitive mutants of bacteriophage mu.
Temperature-sensitive mutants of bacteriophage Mu, which grow at 32 C but not at 42 C, have been isolated. These mutants fall into two groups. Group 1 mutants fail to lyse host cells at nonpermissive temperatures, whereas lysis occurs normally with the group 2 mutants. All of the group 1 mutants apparently belong to the cistrons mapping to the left of gene C, whereas the group 2 mutants have le...
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5, one of the classical T phages of Escherichia coli, has been the subject of many studies, but its genetic analysis has been sporadic. Beginning with heatstable ( s t ) mutants (ADAMS and LARK 1950; ADAMS 1953), there have been reports of mutants with altered host range (LANNI and LANNI 1956), serological behavior (LANNI and LANNI 1957), lysis-inhibiting properties (U. T. LANNI 1958), and buoy...
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gene. in phage, may be defined as the hereditary information determining A the primary structure of a polypeptide chain. In the absence of information concerning mutational alterations of specific proteins, the operational definition of a gene is usually derived from complementation tests. Cells containing two functioning genomes with different recessive mutations in the trans configuration are...
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Certain temperature-sensitive mutants of gene 42 of bacteriophage T4 increase the reversion rates of some rII mutants in the same genome by about 4 to 10 times. This effect was usually found at 34 C, an intermediate permissive temperature, but not at 28 C.
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Temperature shift experiments with temperature-sensitive mutants in T4 gene 43 indicate that the production of a functional T4 DNA polymerase involves the energydependent conversion of an inactive precursor to the active enzyme. Comparisons of the inactive precursor with the active polymerase have demonstrated that the energy-dependent activation affects both the polymerase and 3’ + 5’ exonucle...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1974
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.14.6.1615-1616.1974